Journal: ACS Omega

Article Title: Screening of Methyl-β-cyclodextrins as an Antifading Agent for Cyanine Dye-Labeled Streptavidin to Improve the Performance of Genotyping Chips

doi: 10.1021/acsomega.4c02099

Figure Lengend Snippet: (a) Comparison of fluorescence intensity between unconjugated and SA-conjugated Cy3 dye complexed with MβCD at different MβCD concentrations. (b) Circular dichroism spectra of SA-Cy3 in the presence of MβCD at different concentrations. Fluorescence quenching profile of (c) unconjugated and (d) SA-conjugated Cy3 dye complexed with MβCD.

Article Snippet: The cyanine dye (Cy3 and Cy5) and the streptavidin-conjugated form (SA-Cy3 and SA-Cy5) were provided by Shanghai BioScience Co., Ltd. Methyl-β-cyclodextrin (MβCD) was purchased from Adamas Reagent, Ltd. Polyacrylamide ( M w ∼ 10,000) was purchased from Sigma-Aldrich Co., Ltd. All genotyping chips, custom-made oligonucleotides, and microbeads were provided by LASO Biotech Co., Ltd. (Soochow, China).

Techniques: Comparison, Fluorescence, Circular Dichroism

Journal: ACS Omega

Article Title: Screening of Methyl-β-cyclodextrins as an Antifading Agent for Cyanine Dye-Labeled Streptavidin to Improve the Performance of Genotyping Chips

doi: 10.1021/acsomega.4c02099

Figure Lengend Snippet: (a) Resistance of photobleaching of genotyping chips coated with a film containing 20% MβCD compared to 0% MβCD. (b) Process of MβCD-containing film formation on the genotyping chip surface.

Article Snippet: The cyanine dye (Cy3 and Cy5) and the streptavidin-conjugated form (SA-Cy3 and SA-Cy5) were provided by Shanghai BioScience Co., Ltd. Methyl-β-cyclodextrin (MβCD) was purchased from Adamas Reagent, Ltd. Polyacrylamide ( M w ∼ 10,000) was purchased from Sigma-Aldrich Co., Ltd. All genotyping chips, custom-made oligonucleotides, and microbeads were provided by LASO Biotech Co., Ltd. (Soochow, China).

Techniques:

Journal: ACS Omega

Article Title: Screening of Methyl-β-cyclodextrins as an Antifading Agent for Cyanine Dye-Labeled Streptavidin to Improve the Performance of Genotyping Chips

doi: 10.1021/acsomega.4c02099

Figure Lengend Snippet: Representative scanned images (top) and statistic distribution (column) of grayscale values (bottom) of NP68 beads with imaging buffer and the averaged difference (square) in grayscale values for each level group between buffer and MβCD coating imaging in (bottom left) Cy3 channel and (bottom right) Cy5 channel.

Article Snippet: The cyanine dye (Cy3 and Cy5) and the streptavidin-conjugated form (SA-Cy3 and SA-Cy5) were provided by Shanghai BioScience Co., Ltd. Methyl-β-cyclodextrin (MβCD) was purchased from Adamas Reagent, Ltd. Polyacrylamide ( M w ∼ 10,000) was purchased from Sigma-Aldrich Co., Ltd. All genotyping chips, custom-made oligonucleotides, and microbeads were provided by LASO Biotech Co., Ltd. (Soochow, China).

Techniques: Imaging

Journal: ACS Omega

Article Title: Screening of Methyl-β-cyclodextrins as an Antifading Agent for Cyanine Dye-Labeled Streptavidin to Improve the Performance of Genotyping Chips

doi: 10.1021/acsomega.4c02099

Figure Lengend Snippet: (a) Averaged signal and background grayscale values of each type of NP68 beads. (b) Signal-to-noise ratio of images to be analyzed in each fluorescence channel. The S/N in the Cy3 channel was defined as the ratio of CG bead GS to AT bead GS, and the S/N in the Cy5 channel was defined as the ratio of AT bead GS to CG bead GS. (c) DR values derived from grayscale data for NP68 beads. (d–g′) Representative Cartesian plots for cluster analysis and CSS values for selected Homo sapiens single nucleotide polymorphisms (SNPs): rs1621688 (d), rs379249 (e), rs10852734 (f), and rs11085118 (g) scanned with the imaging buffer, and the corresponding plots scanned with MβCD coatings (d′–g′). R is a log-based normalized grayscale value from both channels and θ = (2/π) × arctan(GS cy3 /GS cy5 ).

Article Snippet: The cyanine dye (Cy3 and Cy5) and the streptavidin-conjugated form (SA-Cy3 and SA-Cy5) were provided by Shanghai BioScience Co., Ltd. Methyl-β-cyclodextrin (MβCD) was purchased from Adamas Reagent, Ltd. Polyacrylamide ( M w ∼ 10,000) was purchased from Sigma-Aldrich Co., Ltd. All genotyping chips, custom-made oligonucleotides, and microbeads were provided by LASO Biotech Co., Ltd. (Soochow, China).

Techniques: Fluorescence, Derivative Assay, Imaging